A Houston-based bus company will pay more than $200,000 to settle Justice Department allegations that it passed over U.S. workers in favor of foreign workers on temporary visas.

The settlement reached with El Expreso Bus Co. is the sixth under the DOJ’s initiative to protect U.S. workers, which was started early in the Trump administration. Through the initiative, the agency has collected more than $425,000 in civil penalties and more than $380,000 in compensation for affected workers.

The DOJ alleged that El Expreso failed to consider U.S. applicants for temporary bus driver jobs and instead hired foreign workers on H-2B...